![]() Mesencephalic flexture Patch#Optogenetics-coupled patch clamp analysis in the NAc demonstrated a significant reduction of glutamatergic neurotransmission, whilst behavioral analysis revealed a normal locomotor sensitization to amphetamine and cocaine. In this study, we implemented a tamoxifen-inducible Cre-LoxP strategy to selectively probe VGLUT2 in mature DA neurons of adult mice. Repeated exposure to cocaine is known to cause lasting adaptations of excitatory synaptic transmission onto medium spiny neurons (MSNs) in the NAc, but the putative contribution of VGLUT2-mediated glutamate co-release from the mesoaccumbal projection has never been investigated. Alterations in DA neurotransmission in the mesoaccumbal pathway has been proposed to contribute to these behavioral alterations but the underlying molecular mechanism remains largely elusive. For example, it has been shown that conditional deletion of Vglut2 gene expression within developing DA neurons in mice causes altered locomotor sensitization to addictive drugs, such as amphetamine and cocaine, in adulthood. 5Service of Neurology, Geneva University Hospital (HUG), Geneva, SwitzerlandĮxpression of the Vglut2/Slc17a6 gene encoding the Vesicular glutamate transporter 2 (VGLUT2) in midbrain dopamine (DA) neurons enables these neurons to co-release glutamate in the nucleus accumbens (NAc), a feature of putative importance to drug addiction.3Department of Neuroscience, Karolinska Institutet (KI), Solna, Sweden.2Department of Basic Neurosciences, University of Geneva, Geneva, Switzerland.1Department of Organismal Biology, Uppsala University, Uppsala, Sweden.Dorst 3, Zisis Bimpisidis 1, Sylvie Dumas 4, Hanna Pettersson 1, Camilla Bellone 2, Gilad Silberberg 3, Christian Lüscher 2,5 and Åsa Wallén-Mackenzie 1* Maria Papathanou 1, Meaghan Creed 2†, Matthijs C. ![]()
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